New research published in the Journal of Nanobiotechnology
Our new manuscript has been published in the Journal of Nanobiotechnology by first author Rachel Mizenko, titled:
"Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers"
In this study we investigate the validity of the conventional method by which many researchers pull-down EVs from complex biofluids for downstream use in cancer diagnostics platforms. Essentially, some pretty common EV membrane proteins, known as tetraspanins, tend to be used to capture EVs from complex biofluids like blood plasma. In the absence of a true global marker that is present on all EVs, tetraspanins have been thought to be a pretty decent handle by which to capture them in high numbers. Our new study shows that the interesting cancer markers also present on EVs don't always appear alongside the tetraspanins. And we show that there are specific combinations of tetraspanins and tumor markers that are "more" co-localized than others. We think this is pretty important to consider for design of EV diagnostic assays looking for these EV tumor markers!
The manuscript is fully open access and all raw data is published alongside the study here. It is also a result of a great collaboration with the Wang Lab here at UC Davis!
Download the pdf of the manuscript directly: